КРАСНЫЕ ЛИСТЬЯ  — ПОЧЕМУ ОНИ ПОЯВЛЯЮТСЯ НА ВИНОГРАДНОЙ ЛОЗЕ, И ЧТО НАДО ПРИ ЭТОМ  ДЕЛАТЬ ВИНОГРАДАРЮ. Делюсь, коллеги,  с Вами двумя материалами ученых из США на эту тему,  ссылки на источники ниже. И немного личных наблюдений.

1.Болезнь виноградной красной пятнистости ( Grapevine Red Blotch Disease) статья авторы Д-р Майсур Р. Сударшана Биолог исследования, USDA-ARS Отдел патологии растений, Университет Калифорнии , Джеймс Вольпер , продюсер, виноградарь.UC Комплексное виноградарство посвящена более частному случаю образования красных листьев в результате вирусной болезни красной пятнистости. ИСТОЧНИК ( язык англ.)  Эта статья небольшая.

2. Красные листья на винограде: биотические и абиотические причины -( Red Leaves in the Vineyard: Biotic and Abiotic Causes) статья Совместный сборник фактографического бюллетеня FS1260 2016 г. Авторы- Хемант Гохиль, аграрный агент ,  Глостер, Мидзухо Нита, ассистент профессора, Политехнический институт Вирджинии и Государственный Университет, Гэри Павлис, аграрный агент, округ Атлантик, Дэниел Уорд, специалист по распространению с/х знаний, специализация помология. Статья более широко раскрывает заданную тему поста и поэтому ниже приводится полностью на русском языке..ИСТОЧНИК (перевод мой, подстрочник технический.) Есть возможность скачать PDF на  англ. языке с рисунками.

Красные листья на винограде. Блог Олёны Непомнящей.

Фото 1 (слева). Калий (K) дефицит, сорт винограда  Каберне Фран. Красные листья, пятна  между жилками.
Фото 2 (в центре). Магний (Mg) дефицит , сорт винограда Шамбурсен. Красные листья, покраснение клиньями начинаются с краев листьев.
Фото 3 (справа). фосфора (P) дефицит, сорт винограда  Зинфандел.. Красные листья, покраснение полей листа.

Красные листья на виноградной лозе появляются по многим причинам – и при этом провоцируются факторами,  как биотическими (вирусами, бактериями и грибками), так и абиотическими (дефицит питательных веществ, морозная травма , повреждение корневой системы, штамба  и т. д.). Все, что может вызвать блокировку или стресс в  проводящей сосудистой системе растения, где перемещается вода и питательные вещества, может привести к красному окрашиванию листьев. Время проявления, внешний вид и распространение могут быть различны. Перекрестное наложение симптомов покраснения листьев затрудняет определение причин, если основываться только на внешних признаках. ( 1) Поэтому лучшая стратегия не в том, чтобы полагаться на догадки, а в том, чтобы как можно скорее произвести анализ виноградной лозы и выяснить точный диагноз .

Ниже приведены примеры абиотических и биотических стрессов, которые чаще приводят к тому, что на виноградной лозе появляются красные листья .

Недостаток питательных веществ

Калий (K) Дефицит К развивается, когда виноградники получают меньше калия, чем то количество, которое требуется им для нормального роста и развития, в частности при низком уровне рН почвы (т.е. менее 5 .0 для Vitis vinifera), что уменьшает доступность калия к корням. Калий является мобильным питательным веществом, и  в случае дефицита в первую очередь получают калий более молодые, а именно верхние листья , а не более старые, нижние. Поэтому сначала появляются красные листья в нижней части куста.

Симптомы обычно проявляются в начале и конце лета.( 2) Листья приобретают красный цвет между жилками, начиная с краев  и по мере того, как дефицит увеличивается, окрашенные листья становятся фиолетовыми (фото 1). На лозах сортов белого винограда на листьях с дефицитом калия из-за недостаточного содержания хлорофилла начинается хлороз ( окрашивание листьев бледно-желтое или белое) .

Чтобы контролировать состояние растения с недостатком калия K, рекомендуется проводить анализ черешков каждые два-три года, желательно, во время цветения. Целевой уровень калия  в черешках должен составлять от 1,5% до 2,5% при отборе во время цветения и от 1,2% до 2,0% при отборе проб через 70 дней после цветения. Применяйте подкормку калием (KOH) либо внекорневую, либо корневую, в зависимости от срочности. При хронической недостаточности калия следует проводить лабораторные испытания почвы на предмет содержания в ней питательных веществ и рН.

Магний (Mg) Листья с недостатком магния могут внешне напоминать калие-дефицитные листья; однако здесь окрашивание начинается с конца листа , создавая клинообразный рисунок (фото 2). Дефицит магния – серьезная проблема у многих красных сортов винограда, и межъядерный хлороз у них проявляется красным или коричневым окрашиванием.. У белых разновидностей  хлороз при дефиците Mg остается желтоватым. Целевой уровень Mg в черешках составляет 0,3% -0,5% при цветении; или 0,35%- 0,75% в пробах через 70 дней после цветения. (2 )Чтобы исправить дефицит магния, применяется магниевая соль (MgSO 4 ), известная как соли Эпсома, но только, если рН почвы не требует корректировок. Если ваша почва кислая, используйте доломитовый известняк (Mg-содержащий известняк), так как он также повысит pH, улучшив скорость поглощения магния.

Фосфор (P). Недостаток фосфора  на листьях красных сортов винограда приводит к кратковременному окрашиванию, в то время как на белоягодных разновидностях винограда проявляется хлороз.(2 ) Исходный хлороз обычно появляется вокруг краев листьев, которые краснеют у красных сортов (фото 3). В отличие от других основных питательных веществ, фосфор обеспечивает большую гибкость с т. зр.сроков применения, поскольку он менее подвижен в почве и не так легко удаляется. Целевой уровень фосфора (P) для черешков листьев должен составлять  0,13 — 0,30% в зависимости от типа подвоев. Моноаммонийфосфат (MAP) и  ди-аммония фосфат(DAP) являются источниками фосфорного удобрения. Скорректируйте  также pH почвы, если он ниже оптимального — для улучшения поглощения фосфора корнями растений..

Болезни

Вирусы винограда, такие как скручивание листьев и красная пятнистость , также могут вызывать появление красных листьев и приводить к понижению жизнеспособности винограда,  понижению урожайности, качества ягод и раннему отмиранию побегов винограда. Так как пока не известны способы борьбы с вирусами и лечения лоз после заражения, единственный способ вылечить зараженный виноградник — это удаление зараженных лоз и посадка посадочного материала из надежных питомников, не зараженные вирусом . (3)

Красные листья на винограде. Блог Олёны Непомнящей.  Красные листья на винограде. Блог Олёны Непомнящей.

Фото 4. Симптомы болезни красной пятнистости листьев виноградной лозы сорта винограда Каберне Совиньон. Обратите внимание на красноватое обесцвечивание на привершинных участках листа,  жилки остаются зелеными, а листья — купированы вниз или скручиваются по краям.

Фото:5. Симптомы болезни , вызванной вирусом скручивания листьев виноградной лозы  сорта винограда Шардоне. Обратите внимание на то, что область листьев в поперечном направлении становится бледно-зеленой, в то время как жилки  становятся желтоватыми, а нижняя часть листа купированной или скрученной по  краям.

Симптомы болезни, вызванной вирусом скручивания листьев виноградной лозы, обычно появляются в течение вегетации ближе к начальной стадии роста и созревания ягод  . У красных сортов винограда окрашивание листьев -от красного до пурпурного- наблюдается на привершинных участках листа,  при этом жилки остаются зелеными (фото 4). У белых сортов винограда привершинные зоны листьев  становятся бледно-зелеными, а жилки- желтоватыми (фото. 5). Кроме того, купирование или скручивание листьев вниз по краям типично для симптомов  вируса скручивания листьев (фото 5). Изменение цвета листьев часто будет развиваться внутри виноградной лозы случайным образом . Восходящее или нисходящее движение обесцвечивания ( точнее, изменения цвета на красный) будет происходить иначе , чем  это происходит при условии  недостатка питательных веществ.

Красные листья на винограде. Блог Олёны Непомнящей.

Фото 6. Виноградные мучнистые червецы (Pseudococcus maritimus) на побеге винограда.

Существует несколько типов вирусов, которые могут вызвать заболевание скручивания листьев, это вирусы-липиды-1 и -3 (GLRaV-1 и -3), которые могут распространяться мучнистыми червецами или другими сосущими насекомыми (фото  6). Мониторинг вредоносного порога этих векторных насекомых нужно делать в течение сезона (в период до, во время и после цветения), внимательно исследуя базальную область зеленых побегов и под корой. С мучнистыми червецами можно бороться с помощью инсектицидов, однако недавние исследования показали, что даже малое количество мучнистых червецов может быстро распространить вирус скручивания листьев на винограднике.

Симптомы вируса красной пятнистости на винограде

Красные листья на винограде. Блог Олёны Непомнящей.

Фото 7. Симптомы вируса красной пятнистости, сорт винограда Мерло. Обратите внимание, что красный пигмент появляется на листе в случайном порядке. Покраснение краев можно ошибочно принять за недостаток калия, а скатывание вниз краев листьев можно ошибочно принять за симптомы вируса скручивания листьев.

Возбудитель болезни красной  пятнистости винограда   это относительно недавно обнаруженный вирус, который , тем не менее, существует в течение длительного времени. Как следует из названия, пятна красного пигмента появляются случайным образом на листьях зараженных виноградных лоз. Он может быть обнаружен на любой стадии роста виноградного растения  и в любой части лозы. Его можно легко ошибочно принять за недостаток калия или вирус скручивания листьев , особенно когда на лозе листья с опущенными вниз краями  или скручены (рис. 7). Из-за недавней идентификации вируса предпринимаются постоянные усилия, чтобы понять, как (или если) он распространяется на виноградниках. Известно, что вирус красной пятнистости может быть распространен путем прививки зараженного привоя или на зараженный подвой или другим способом использования зараженного посадочного материала.

Для двух вирусов, упомянутых выше, и любых других вирусов, лозы всегда будут заражены. Таким образом, при создании виноградника рекомендуется получать только материалы, проверенные на вирусы, и из проверенных  питомников. Убедитесь, что в школке установлены процедуры тестирования на вирусы, такие как Протокол 2010. 4 Материалы из Протокола 2010 доступны с 2013 года, а возможность покупки увеличивайте по мере того, как большее количество  питомников адаптировало протокол.

Если вы производите свой посадочный материал со своих  реально существующих виноградников или сами получили материалы из не сертифицированных питомников, подумайте о том, чтобы отправить репрезентативное количество черенков для проверки на вирусы перед посадкой. Как правило, 7- 8 молодых зрелых листьев с лозы и 30-50 листьев с виноградника достаточно, но лучше уточнить у лаборатории тестирования вирусов их конкретный протокол выборки. Когда в вашем винограднике присутствует хоть один из векторных насекомых или один из вирусов, связанных с липидами виноградной лозы (GRLaV-1 или -3), для того, чтобы сохранить здоровые виноградной лозы, потребуется борьба с векторными насекомыми.с GLRaV.

Корончатый галл «Crown Gall» , известный нам как Бактериальный рак БР» (разрастание рыхлой ткани растений). вызван бактерией Rhizobium (= Agrobacterium ) vitis и распространен в прохладных широтах. Холодные зимы или ранневесенние колебания температуры повреждают кору и проводящие сосудистые ткани, а также позволяют R. vitis переместиться в клетки винограда и вызвать инфекцию. Типичными симптомами являются образование галлов на стволе (фото 8); однако эти галлы могут быть очень маленькими и могут образовываться под корой (фото9). Так, БР можно ошибочно идентифицировать как недостаток питательных веществ, так как легко заметны красные листья, полученные из-за стресса проводящей сосудистой системы. (фото 10).

Красные листья на винограде. Блог Олёны Непомнящей.  Красные листья на винограде. Блог Олёны Непомнящей.Фото 8. Симптомы бактериального рака в месте прививки. БР на побеге винограда

Фото 9. Ряд мелких галлов БР, образованных под корой, сорт винограда Мерло.

Необходимо закладывать виноградники свободным от бактериального рака посадочным материалом. Другими превентивными мерами являются ведение кустов с несколькими штамбами, чтобы в случае поражения раковым наростом одного штамба можно было бы сделать замену без потери урожайности, и окучивание места прививки в зимние месяцы.
Что же делать при подозрении на заражение кустов. Другие операции, такие как механическое повреждение штамба оборудованием ( например, триммерами), также могут способствовать заражению лоз Rhizobium (= Agrobacterium ) vitis . Как и при вирусных заболеваниях, зараженные БР лозы заражаются на всю жизнь, потому что бактерии , источники БР, являются системным патогеном.  Таким образом, управлять БР сложно. Для получения не зараженного посадочного материала обратите внимание на процедуру, используемую в Протоколе 2010, которая должна значительно снизить риск возникновения вируса R. vitis у материнского растения, и не забудьте выбрать сорта, соответствующие вашим требованиям. Сорта для прохладного климата, такие как Мерло и Танат, как правило, имеют более высокий риск заражения БР, чем многие другие. Правильные методы виноградарства, такие как выбор места, контроль урожайности и болезней, могут снизить риск развития БР или корончатого галла.. Другими превентивными стратегиями являются такие-как ведение на лозе нескольких штамбов с возможностью подстраховки ( замены ) и окучивание  прививки в зимние месяцы. Подробнее ЗДЕСЬ информация и статья о бактериальном раке (или корончатом галле) . .

Красные листья на винограде. Блог Олёны Непомнящей.

Фото 10. Красные листья, образовавшиеся из за корончатого галла (БР) . Обратите внимание на образование галлов БР на стволе.

 

 

 

 

 

Оценка внушающих подозрение виноградных лоз и что потом делать с ними.

Трудно точно определить причину красного окрашивания листьев.  Тесты на вирусы (красной пятнистости, скручивания листьев и др.) являются дорогими по цене, поэтому диагностику разумнее вести по последовательному пути.

1. первая рекомендация в блок-схеме идентификации в том, чтобы максимально быстро определить повреждены или нет корневая система, штамб и плечи куста, то есть  проверить целостность проводящей сосудистой системы, Если да, целостность нарушена, то решать эту проблему.

2.  если проводящая сосудистая система куста не повреждена, то следует затем  выполнить анализ черешков (рис. 11) и выяснить есть ли недостаток калия, магния, фосфора. Если  да, то решать эту проблему.

3. Если исключаются повреждения штамбов , проводящей системы и  дефицита питательных веществ тоже нет, то в этом случае образцы должны быть отправлены на тестирование на вирусы.

Красные листья на винограде. Блог Олёны Непомнящей.Фото 11.( рис.). Блок-схема действий, которые необходимо предпринять после того, как вы заметили красные листья на своем виноградной лозе. A flowchart for actions to be taken after you observed red leaves on grapevines.

 

 

Фотографии 1- 3 Хеманта Гохиля, Фотографии  4-6, 8-10  Mizuho Nita,Фото  7  Prashant Swamy.  В приложении к статье приведен список лабораторий тестирования вирусов виноградной лозы.

Цитированные ссылки 1 Марк Фукс. 2013. Новости и информация о программах на Фондовых заводских услугах. 2 Тони Вольф (ред.) 2008. Руководство по производству виноградных вин для Восточной Северной Америки, издательство по производству растений и быта. NRAES 145 3 Хемант Гохил, Гэри Павлис и Даниэль Уорд. 2015. Ратгерс NJAES Совместное расширение фактографического бюллетеня FS1252, 4 Адиб Роухани и Дебора Голино. 2010. Протокол испытаний вируса виноградной лозы 2010. Бюллетень программы FPS Grape Program, октябрь 2010 г. 5 Стивен Васкес и Джоди Креасап Джи. 2013. Crown Gall: в третьем издании Grape Pest Management издание UCANR. Pp. 71-74

Перспективы тестирования  на вирусы в России. Вышеприведенные статьи ученых США  интересны. Но возникает закономерный вопрос , а можно ли проверить виноградную лозу на патогены виноградарям в России ( причем не только на патогены , связанные с красным окрашиванием  листьев, но и на другие вирусы и бактерии) . Известно, что на больших виноградных плантациях есть тепличное, полевое и лабораторное тестирование. Есть  и собственные лаборатории, где проводятся тесты, к примеру серодиагностика ( ELISA – латекс-тест, тест электронной микроскопии). При помощи (полимеразной цепной реакции — ПЦР ). или микробиологического метода возможно обнаружить у кустов-клонов скрытую инфекцию возбудителем бактериального рака.  И наконец есть проект института Магарач  и Федерального исследовательского центра “Фундаментальные основы биотехнологии”, который направлен на то, чтобы разработать праймеры, специфично реагирующие на  конкретные вирусы и бактерии. Это компоненты тест-системы, которая создается в рамках проекта. Эта система будет включать набор реактивов, необходимых для определения вируса или бактерии винограда. Предполагается, создание несколько наборов для определения различных возбудителей. К примеру,  есть неблагополучные признаки на винограднике, и нужно поставить диагноз, узнать, что с ним. Особенно  разумно это делать при закладке новых виноградников. Но пока непонятно , смогут ли эту тест-систему использовать обычные агрономы и виноградари, или это проект только для людей, обладающих соответствующими  знаниями и навыками. Или  вообще это только работа для лабораторий со специализированным научным оборудованием , где лаборант делает анализ и проверяет. На настоящий момент  проверить одно растение на наличие патогенов методом ПЦР стоит  примерно около 2 тысяч рублей.Источник 

Ремарка моя. На нашем тверском винограднике красные листья пока отмечаются  периодически  на сортах винограда Регент и ПГ-116. Симптомы сходны с недостатком магния. Для устранения дефицита использовалась листовая подкормка калимагнезией.

Кроме того, в 2015 году был факт случайного повреждения триммером  многолетнего рукава сорта  винограда Ключевской. Тогда в течение двух дней  листья  на большинстве побегов этого рукава приобрели красный цвет. Поврежденный фрагмент лозы был удален, произведена подкормка фосфором и калием, использован антистрессовый препарат Кендал.

С уважением, Олёна Непомнящая.

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